Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and hearts were dissected (n=4–20 per group; black columns: vehicle, gray: 1mg HC, hatched: 5mg HC, dotted: 10mg HC). Fulton’s index was measured as the weight of the right ventricle (RV) divided by the weight of the left ventricle plus septum (LV+S). Data are shown as mean ± SEM, *p<0.05 vs. normoxia-vehicle, †p<0.05 vs. hyperoxia-vehicle.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques:

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and lungs were inflation fixed (n=5–16 per group). A) Pulmonary hypertension was assessed by medial wall thickness (MWT) of small PAs (black columns: vehicle, gray: 1mg HC, hatched: 5mg HC, dotted: 10mg HC). B) Representative H&E-stained lung sections (magnification 40X) for normoxia-vehicle, normoxia+1mg HC, hyperoxia-vehicle, and hyperoxia+1mg HC mice. Data are shown as mean ± SEM, *p<0.05 vs. normoxia-vehicle, †p<0.05 vs. hyperoxia-vehicle. Bar = 50 μm.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques: Staining

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and lungs were inflation fixed (n=5–17 per group). Hematoxylin-stained sections were imaged at 20X, and A) mean alveolar area (black columns: vehicle, gray: 1mg HC, hatched: 5mg HC, dotted: 10mg HC) and B) mean chord length (black columns: vehicle, gray: 1mg HC, hatched: 5mg HC, dotted: 10mg HC) were measured using Scion software. Data are shown as mean ± SEM, *p<0.05 vs. normoxia-vehicle, †p<0.05 vs. hyperoxia-vehicle.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques: Staining, Software

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and lungs were inflation fixed. Hematoxylin-stained sections were imaged at 20X. Representative hematoxylin-stained lung sections are shown for normoxia-vehicle (panel A), normoxia+1mg HC (panel B), normoxia+10mg HC (panel C), hyperoxia-vehicle (panel D), hyperoxia+1mg HC (panel E), and hyperoxia+10mg HC mice (panel F). Bar = 200 μm.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques: Staining

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and lungs were inflation fixed. Lung sections were stained with the Verhoeff-van Gieson stain and imaged at 40X. A) Representative elastin-stained lung sections are shown for normoxia-vehicle, normoxia+1mg HC, normoxia+10mg HC, hyperoxia-vehicle, hyperoxia+1mg HC, and hyperoxia+10mg HC mice. Bar = 25 μm. B) Elastin was calculated as percentage of elastin area/percentage tissue area.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques: Staining

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and body weight was obtained (n=7–25 per group; black columns: vehicle, gray: 1mg HC, hatched: 5mg HC, dotted: 10mg HC). Data are shown as mean ± SEM, *p<0.05 vs. normoxia-vehicle, †p<0.05 vs. hyperoxia-vehicle.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques:

Journal: Pediatric research

Article Title: Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury

doi: 10.1038/pr.2016.1

Figure Lengend Snippet: A) Mice exposed to 75% O 2 (hyperoxia) or room air (normoxia) ± hydrocortisone (1mg HC, 5mg HC, or 10mg HC) or sterile water (vehicle) were euthanized and PA protein was harvested. PDE5 specific activity was measured as the sildenafil-inhibitable fraction of total cGMP hydrolysis and normalized for total milligram of protein (n=5–9 per group, black columns: vehicle, white: 1mg HC). Data are shown as mean ± SEM, *p<0.05 vs. normoxia-vehicle, †p<0.05 vs. hyperoxia-vehicle. B) PASMC isolated from healthy 14d neonatal mice were exposed to 95% O 2 -5% CO 2 or 21% O 2 -5% CO 2 for 24h and total protein was harvested. PDE5 specific activity was measured as the sildenafil-inhibitable fraction of total cGMP hydrolysis and normalized for total milligram of protein (n=5–7 per group; black columns: untreated, white: 100nM HC). Data are shown as mean ± SEM, *p<0.05 vs. 21% untreated, †p<0.05 vs. 95% untreated.

Article Snippet: Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth ( , ).

Techniques: Activity Assay, Isolation